GATEWAY CLONASE II MANUAL

[PDF]
Gateway Technology with Clonase II - Thermo Fisher Scientific
The Donor Vector box, the Gateway® BP Clonase®II Enzyme Mix and Reagents box, and the M13 Sequencing Primers box (Boxes 1–4) contain the following items. Store the Gateway® BP Clonase®II enzyme mix at −30°C to −10°C for up to 6 months. For long-term storage, store at −85°C to −68°C.File Size: 1MBPage Count: 74People also askWhat do you need to know about gateway LR Clonase II?What do you need to know about gateway LR Clonase II?Gateway® LR Clonase® II contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set-up with fewer pipetting steps. For Research Use Only.Gateway™ LR Clonase™ II Enzyme mix - Thermo FisherSee all results for this questionWhat is the function of the gateway BP Clonase enzyme?What is the function of the gateway BP Clonase enzyme?Gateway BP Clonase enzyme contains both Int (Integrase) and IHF (Integration Host Factor) proteins that catalyze the in vitro recombination of PCR products or DNA segments from clones (containing attB sites) and a Donor vector (containing attP sites) to generate Entry clones.Clonase Enzymes | Thermo Fisher Scientific - USSee all results for this questionHow to make Invitrogen BP Clonase II enzyme mix?How to make Invitrogen BP Clonase II enzyme mix?Thaw on ice the Invitrogen BP Clonase II enzyme mix for about 2 minutes. Vortex the BP Clonase II enzyme mix briefly twice (2 seconds each time). To each sample (Step 1, above), add 2 µl of BP Clonase II enzyme mix to the reaction and mix well by vortexing briefly twice.Gateway Cloning Protocols | Thermo Fisher Scientific - USSee all results for this questionHow to store gateway Clonase?How to store gateway Clonase?Store the Gateway® BP Clonase®II enzyme mix at −30°C to −10°C for up to 6 months. For long-term storage, store at −85°C to −68°C. Store all other components at −30°C to −10°C. Store Zeocin™Selection Reagent at −30°C to −10°C, protected from light.Gateway Technology with Clonase II - Thermo Fisher ScientificSee all results for this questionFeedback[PDF]
Gateway BP Clonase II Enzyme Mix - Thermo Fisher Scientific
attL × attR reaction is mediated by Gateway ® LR Clonase ™ II enzyme mix. ccdB is the F plasmid-encoded gene that inhibits growth of E. coli (2,3) and “gene” represents any DNA segment of interest (e.g. PCR product, cDNA, genomic DNA). Description Gateway® BP Clonase™ II enzyme mix is a proprietary enzyme and buffer[PDF]
Gateway LR Clonase II Enzyme Mix Product Information Sheet
LR Clonase™ II enzyme mix is supplied as a 5X solution. If you wish to scale the reaction volume, make sure the LR Clonase™ II enzyme mix is at a final concentration of 1X. For a positive control, use 100 ng (2 μL) of pENTR™-gus. 1. Add the following components to a 1.5-mL microcentrifuge tube at room temperature and mix:File Size: 154KBPage Count: 4[PDF]
Gateway LR Clonase II Plus Enzyme Mix
Gateway® LR Clonase™ II Plus enzyme mix contains the bacteriophage lambda recombination proteins Integrase (Int) and Excisionase (Xis), and the E. coli - encoded protein Integration Host Factor (IHF) (1), and reaction buffer provided[PDF]
E. coli Expression System with Gateway Technology
For more detailed information about Gateway ® Technology, refer to the Gateway ® Technology with Clonase ® II manual. To generate an entry clone, refer to the manual for the entry vector you are using. The Gateway ® Technology with Clonase ® II manual and entry vector manuals are available for downloading from
Gateway™ LR Clonase™ II Enzyme mix
Gateway® LR Clonase® II contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set-up with fewer pipetting steps. For Research Use
Gateway™ BP Clonase™ II Enzyme mix - thermofisher
Gateway® BP Clonase® II contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set up with fewer pipetting steps. For Research Use Only. Not for use in
Clonase Enzymes | Thermo Fisher Scientific - US
Gateway LR Clonase II enzyme mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor) and Xis (Excisionase) enzymes that catalyze the in vitro recombination between an Entry clone (containing a gene of interest flanked by attL sites) and a Destination vector (containing attR sites) to generate your expression clone.
Gateway Cloning Protocols | Thermo Fisher Scientific - US
Thaw on ice the Invitrogen LR Clonase II enzyme mix for about 2 minutes. Vortex the LR Clonase II enzyme mix briefly twice (2 seconds each time). To each sample (Step 1, above), add 2 µl of LR Clonase II enzyme mix to the reaction and mix well by vortexing briefly twice.
Gateway Cloning | Thermo Fisher Scientific - US
Single-step BP/LR Clonase reaction protocol. DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money. Reduce the overall time needed to obtain an Expression clone from 3 days to 1; Reduce the cost by using less BP Clonase enzyme; This protocol has been published in BioTechniques
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